Confusion arose over where it should be placed and whether it should be broken.
The statement from the oversight body outlined that the yellow band is mandatory to all matatus with a passenger capacity of 25 persons and below.
In addition, NTSA quoted a Legal Notice in which the "Michuki Rules" required that the yellow line should be broken and run through the back of the vehicle as well.
According to the NTSA statement, the band should be visible from a distance of 275 metres.
Why 'Nganyas' are Exempted
"All Matatus with a seating capacity of 25 and below should, therefore, be painted in a broken Horizontal Yellow Band," the notice read in part.
Legal Notice No.65 of 2005 states: "Every taxicab or matatu shall have painted on both sides and on th rear a broken horizontal yellow band having a width of 150 milimetres and of a consistency sufficient to enable such band be clearly visible by a distance of not less than 275 metres."
Another controversial issue that boggled the minds of PSV owners and members of the public was the drawing of graffitti on the vehicles.
NTSA Director-General Francis Mejja clarified that grafitti is allowed so long as the name of the matatu Sacco is clearly visible and the grafitti does not cover windows.
"No innovation decorations should be painted, sprayed and drawn on or affixed to any window, front and back windscreens, lights, indicators or chevrons of the vehicle," Mejja's statement clarified.