Gonzalo Higuain and Federico Bernardeschi will support Portuguese star Ronaldo in a 4-3-3 lineup with Dybala out of the starting line-up with a left thigh injury.
Ronaldo was rested in Juventus' final game of the season with Maurizio Sarri's side having wrapped up a ninth consecutive Serie A title with two matches to spare.
Lyon coach Rudi Garcia has opted for Karl Toko-Ekambi over Moussa Dembele to start up front alongside captain Memphis Depay, who has recovered from a serious knee injury, in a 3-5-2 lineup.
Lucas Tousart's goal gave Lyon a surprise 1-0 win in February, but the French midfielder is now at Hertha Berlin.
Juventus (ITA) 4-3-3
Wojciech Szczesny; Juan Cuadrado, Matthijs De Ligt, Leonardo Bonucci, Alex Sandro; Rodrigo Bentancur, Miralem Pjanic, Adrien Rabiot; Federico Bernardeschi, Gonzalo Higuain, Cristiano Ronaldo
Coach: Maurizio Sarri (ITA)
Lyon (FRA) 3-5-2
Anthony Lopes; Jason Denayer, Marcelo, Fernando Marcal; Leo Dubois, Houssem Aouar, Bruno Guimaraes, Maxence Caqueret, Maxwel Cornet; Karl Toko-Ekambi, Memphis Depay
Coach: Rudi Garcia (FRA)
Referee: Felix Zwayer (GER)